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Characterization and lytic activity of Pseudomonas fluorescens phages from sewage BJM
Radhakrishnan,Ananthi; Ananthasubramanian,M.
Pseudomonas fluorescens phages from sewage were tested against P. fluorescens isolates of soil and sewage. The phages were characterized as to host range, morphology, structural proteins and genome fingerprint. Of the seven phages isolated, one was found to be abundant in sewage (5.9×10(7) pfu/mL), having broad host range, and distinct protein and DNA profile when compared to the other six phages. DNA restriction and protein profiles of the phages and their morphology indicate the diversity in the sewage environment. None of the isolates from the rhizosphere regions of various cultivated soils were susceptible to phages isolated from sewage.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas fluorescens; Phages; RFLP; AFM; Protein analysis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100042
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Inadequacy of bacterial indicators for assessing elimination rates of viruses from molluscan shellfish ArchiMer
Burkhardt, W; Watkins, W; Rippey, S.
The reported incidence of shellfish-borne illness in the United States increased dramatically during the last decade. Most of the outbreaks are attributed to diseases of viral aetiology. These human health problems result primarily from the ingestion of raw shellfish which have accumulated enteric pathogens from environmental waters or from wet storage facilities. Our studies have demonstrated that hard-shelled clams concentrate different indicator micro-organisms at variable and unpredictable rates during the year. In temperate waters, this has been observed at two abbreviated periods in mid-spring and again in late fall when accumulation rates increased dramatically. Moreover, these rates were not generally coincident for viruses and bacteria. In...
Tipo: Text Palavras-chave: Mollusca; Intestinal microflora; Phages; Seafood; Public health; Diseases; Marine molluscs; Viruses; Shellfish; Self purification; Bacteria; Indicator species.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1609.pdf
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Use of rotavirus virus-like particles as surrogates to evaluate virus persistence in shellfish ArchiMer
Loisy, Fabienne; Atmar, R; Le Saux, Jean-claude; Cohen, J; Caprais, Marie-paule; Pommepuy, Monique; Le Guyader, Soizick.
Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log(10) of VLPs was depurated after 1 week in warm seawater (22 degrees C). Phage MS2 was depurated more rapidly (about 2 log(10) in 1 week) than were VLPs, as determined using a single-compartment model and linear regression analysis. After being relayed in the estuary under the influence of the tides, VLPs were detected in oysters for up to 82 days following seeding with high levels of VLPs (concentration range between 10(10) and 10(9) particles per g of pancreatic tissue)...
Tipo: Text Palavras-chave: Seawater; Viral diseases; Bioaccumulation; Depuration; Contamination; Model; Pancreas; Regression analysis; Shellfish; Phages; Virus.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1233.pdf
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